Wheat DArT^®

Triticarte’s wheat DArT first service has been based on a survey of about 16,000 clones from a PstI(TaqI) genomic representation of hexaploid wheat.

2,500 markers polymorphic in a wide range of Australian and European wheat cultivars were discovered in that genomic representation and were used in Triticarte’s service, Wheat PstI(TaqI) v2.3.

A survey of about 19,000 clones from a PstI(TaqI) genomic representation of a wide range of Triticum species of various ploidy resulted in the discovery of about 2,500 markers polymorphic in a wide range of Triticum germplasm. Combined with the markers of version 2.3, these markers were used in Triticarte service Wheat PstI(TaqI) v2.6.

To increase coverage of the D genome of bread wheat, both services were upgraded in September 2009 by adding markers discovered in a PstI(TaqI) genomic representation of D genome enriched DNA fractions prepared by Jaroslav Dolezel’s laboratory at the Institute of Experimental Botany of Olomouc in the Czech Republic (Wenzl et al., 2009 submitted). Standard density array is service v2.3_D and high density array is service v3.

A whole-genome integrated map for a cross between wheat cultivars Cranbrook and Halberd using 339 RFLP, SSR and AFLP markers, 71 STM markers and 339 DArT markers is described in the 2006 paper, "Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome" by Akbari et al. in Theoretical and Applied Genetics,, DOI 10.1007/s00122-006-0365-4.

An alignment of the maps of 9 crosses is now available. The Read Me document describes the content and rationale: this (Version 1.2) is a work in progress. For more information contact peter  DiversityArrays.com