Barley DArT^®

Triticarte’s first barley DArT service is based on a survey of about 10,000 loci for two different genomic representations from cultivated barley. The PstI(BstNI) genomic representation contains about 1,500 markers polymorphic in a wide range of Australian and European barley cultivars. Triticarte’s service, Barley PstI(BstNI) v2.0, produces the genotypes for these markers.

A higher density array is available as Barley PstI(BstNI) v1.7. It comprises the markers of v2.0 plus an additional 1,000 markers discovered in wild barley accessions.

Service Barley PstI(BstNI) v3.0 under development will assay all unique sequenced markers available.

Service Barley PstI(BstNI) vX.0, under development, will provide the genotypes of approximately 400 unique, sequenced markers, most of them mapped on the integrated genetic map of barley.

For a higher density of markers, the second genomic representation could be developed into a new service with approximately the same number of markers. Triticarte Pty Ltd will assess the need and demand for the service.

High-density Consensus Map of Barley

For a description of our "high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits" by P. Wenzl et al., 2006, see BMC Genomics 2006, 7:206 <www.biomedcentral.com/1471-2164/7/206> The map was assembled from seven DH and three RIL individual maps using PhenoMap® (GeneFlow Inc.) and JoinMap 3.0.

Triticarte^® First Linkage Map for Barley

• Integrated with best barley RFLP map (Steptoe x Morex)
• Nearly 1,000 polymorphic DArT^® markers
• Complete coverage and good distribution
• Details published in PNAS June 2004 (www.pnas.org/cgi/content/abstract/0401076101v1)