Frequently Asked Questions

Q: How to prepare DNA suitable for DArT?
A: DArT requires DNA suitable for restriction enzyme digestion and ligation. A recommended protocol for DNA extraction from leaf tissues is available here ( 130 KB).

Q: How much DNA do you need?
A: We need about 10 to 20 ul of 50 ng/ul high quality genomic DNA, suitable for restriction enzyme and ligation.

Q: How to prepare a sample tracking file for ordering the service?
A: Please read the Help file of the ordering system, available here.

Q: How to ship the DNA?
A: Please see the DNA shipping instructions sheet: shipping instructions ( 263 KB)

Q: Are there any charges additional to the price indicated on the online ordering system?
A: For overseas (out of Australia) customers, we recover the cost of importing the DNA through a DNA import charge of 100 AUD per shipment. If the DNA fails our Quality Control and a new plate is sent, we charge 200 AUD for the repeat handling and QC. We encourage all users to control the quality of the DNA samples carefully, as instructed on the Service Specification, before shipping the DNA ! If you elect to pay by credit card, we will add a credit card surcharge of 3.5%. For details, please read the service description page and a service specification sample.

Q: How long does it take to get the data back?
A: We are committed to produce data within 8 weeks of the DNA passing QC. If we get delayed, you will be notified.

Q: How much does the service cost?
A: Details of the available services are on the service description page. You may also use the quotation function from the online ordering system.

Q: What to do if I cannot buy the recommended plates for shipping DNA?
A: We can sell you a set of 10 recommended plates and caps, for 200 Australian Dollars.

Q: Do you have Bulk Segregant Analysis service?
A: Yes! See the details on the service description page.

Q: Is the sequence of DArT markers available?
A: The sequence of many DArT markers has been determined and is currently available to Triticarte customers under the terms of a Sequence Transfer Agreement ( 450 KB). All sequences will be eventually released on a public database and available without restriction.

Q: Do you offer service for other species than Wheat and Barley?
A: Our sister company Diversity Arrays Technology Pty Limited offers services for more than 20 other species. See www.DiversityArrays.com for more details.